Research Technician in Spiegelman Laboratory

Job Details

Job ID:

450 Brookline Ave, Boston, MA 02215

Research Laboratory

Employment Type:
Full time

Work Location:
Onsite: 100% onsite


This position in Dr. Bruce Spiegelman’s Lab will assist postdocs in performing scientific research aimed at understanding the mechanistic basis of metabolic disease. The research technician will collaborate with other scientists and team members, working independently as well as part of multi-disciplinary research teams to design and execute experiments for ongoing research projects. Duties include working with laboratory mice, conducting basic molecular and cell biology, and implementing mass spectrometry for proteomic analysis. Procedures include but are not limited to: isolation and analysis of DNA, RNA, and protein from cultured cells and animal tissues; mass spectrometry sample preparation and data analysis; and primary cell culture and mouse handling and experimentation. There will be extensive opportunities for intellectual engagement with data analysis, experimental planning, oral and written scientific communication, as well as exposure to cutting-edge experimental methods; the position will be a good stepping stone for those interested in a biomedical research career.

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to create an inclusive, diverse, and equitable environment where we provide compassionate and comprehensive care to patients of all backgrounds, and design programs to promote public health particularly among high-risk and underserved populations. We conduct groundbreaking research that advances treatment, we educate tomorrow's physician/researchers, and we work with amazing partners, including other Harvard Medical School-affiliated hospitals.


  • Assisting with general laboratory maintenance and upkeep.
  • Assisting postdocs and students with cell based assays, including maintenance of cell lines, isolation of primary cells from mouse tissue, and genetic manipulation including CRISPR knockout and viral transduction.
  • Assisting with biochemical techniques such as protein purification and labeling, protein-protein or protein-small molecule interactions, enzyme assays,and metabolite measurements.
  • Assisting with molecular biology techniques such as cloning and plasmid purification, as well as isolation and analysis of RNA, DNA, and protein from mouse tissue and cellular samples.
  • Maintaining of animal (laboratory mouse) colonies, including weaning, tail biopsies, DNA preparation and genotyping by PCR.
  • Assisting with animal experiments including mouse phenotyping, intraperitoneal (IP) injection and intravenous (IV) injection, animal sampling and dissection, and tissue harvesting.
  • Preparation of samples for mass spectrometry analysis.


  • Bachelor of Science or Bachelor of Arts degree with scientific specialty is required (or to be obtained in Spring 2024).
  • Previous experience working in a scientific research setting, with mass spectrometry experience preferred.
  • Knowledge of information and techniques related to biology, chemistry, or biophysics is preferred.
  • Must be a self-motivated and independent worker with excellent written and verbal communication skills.
  • Must be strong critical thinker with attention to detail and thorough in completing, troubleshooting, and communicating the results of work tasks.

At Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong.  As relentless as we are in our mission to reduce the burden of cancer for all, we are equally committed to diversifying our faculty and staff.  Cancer knows no boundaries and when it comes to hiring the most dedicated and diverse professionals, neither do we. If working in this kind of organization inspires you, we encourage you to apply.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other characteristics protected by law.

EEOC Poster

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